8-Xylosidase mutant immobilization on UiO-66-NH2 for continuous production of ginsenoside Rg1 and selective production of furfural from notoginsenoside R1

作者：Li, Na; Xia, Huan; Liu, Shiyuan; Teng, Junjiang^*; Jiang, Yanbin^*

来源：INDUSTRIAL CROPS AND PRODUCTS, 2023, 197: 116563.

DOI：10.1016/j.indcrop.2023.116563

• Ginsenoside Rg1
• Furfural
• 8-xylosidase
• Immobilization
• Metal -organic framework

In order to improve the activity and reusability of 8-xylosidase, the 8-xylosidase mutant S160N-R333H (R333H) which could convert notoginsenoside R1 to ginsenoside Rg1 and xylose was immobilized on the metal-organic framework (MOF) UiO-66-NH2. The physical and biochemical properties of immobilized 8-xylosidase (R333H@UiO-66-NH2) were studied in detail. The results showed improved performances, i.e. the high immo-bilization yield of 93% and recovery activity of 192.8%, better stability under acidic or trypsin-containing conditions, the higher activity in the reaction containing ions, and the improved catalytic efficiency (was 1.37-fold than that of free R333H). Importantly, the ginsenoside Rg1 yields remained more than 80% after six recycles in McIlvaine buffer and pure water. The UiO-66-NH2 could catalyze xylose dehydration to produce furfural and the yield reached 69%. Thus this study provides a promising method for production of bioactive or platform chemicals via continuous conversion of xyloylated substrates using nanocatalyst synthesized by 8-xylosidase and MOF.

• 单位
  茂名学院