科研之友
微信
新浪微博
Facebook
分享链接
摘要
OBJECTIVE: With this study, we aimed at exploring the regulation mechanism of Potentilla discolor-Euonymus alatus on intesti-nal flora of T2DM (Type 2 Diabetes Mellitus) rats induced by high-fat diet combined with strepto-zotocin.MATERIALS AND METHODS: T2DM rats were induced by high-fat diet combined with strepto-zotocin. There were normal control group, mod-el group, metformin group, high-dose Chinese medicine group and low-dose Chinese medi-cine group. Each group included 10 rats. Normal control group: normal feeding, no modeling, or-dinary feed, and gavage of 0.9% normal saline. Model group: T2DM rats, high-fat diet, and ga-vage of 0.9% normal saline. Metformin group: T2DM rats, high-fat diet and fed with metformin solution. High-dose Chinese medicine group: T2DM rats, high-fat diet, and gavage of concen-trated Chinese medicine at a dose of 6 times the clinical dose. Low-dose Chinese medicine group: T2DM rats, high-fat diet, and gavage of concentrated Chinese medicine at a dose twice the clinical dose. The general situation of T2DM rats was observed, and the changes of intestinal flora were observed with 16SrDNA sequencing.RESULTS: The T2DM rats induced by high-fat diet combined with streptozotocin were mold-ed. After intervention, at the class level, the ra-tio of gamma-proteobacteria was 22.30% in the model group, 11.97% in the metformin group, 3.24% in the high-dose Chinese herbs group and 1.72% in the low-dose Chinese herbs group; the ratio of Erysipelothrix insidiosa was 4.73% in the model group, 4.68% in the metformin group, 3.93% in the high-dose Chinese herbsgroup and 2.92% in the low dose group; the ratio of Lactinobacillus was 2.30% in the model group, 0.01% in the met-formin group, 0.00% in the high-dose Chinese herbs group, and 0.00% low-dose Chinese herbs group; at the portal level, the Firmicutes/Bacte-roides was 0.88 in the normal control group, 3.40in the model group, 1.71 in the metformin group, 2.74 in high-dose Chinese medicine group, and 1.34 in low-dose Chinese medicine group; at the genus level, the relative abundance of Lactoba-cillus in the model group was 3.28%, that of Ak-kermansia was 1.99%, that of Shigella coli was 22.08%, and that of Vibrio phaseus was 7.67%. All of them were improved after the intervention of metformin and traditional Chinese medicine.CONCLUSIONS: Potentilla discolor-Euony-mus Alatus could improve the composition and structure of intestinal flora in T2DM rats and reg-ulate the diversity of intestinal flora. The ratio of Firmicutes/Bacteroidetes was adjusted, mainly to increase the number of Bacteroides; the flora related to intestinal barrier was adjusted, mainly to increase the number of Lactobacillus and Ak-kermansia bacteria.
-
单位广东药学院; 广州医学院; 1
