The antifeedant and antifungal cryptic metabolites isolated from tobacco endophytes induced by host medium and coculture

作者：Chen, Jing-Xin; Xia, Dan-Dan; Yang, Xue-Qiong^*; Yang, Ya-Bin; Ding, Zhong-Tao^*

来源：FITOTERAPIA, 2022, 163: 105335.

DOI：10.1016/j.fitote.2022.105335

• Nicotiana tabacum L
• Endophyte
• Cryptic metabolite
• Induction
• Antifungal activity
• Antifeedant

Four new cryptic metabolites including one fumagillol derivative (2), one cyclohexenone derivative (4), one 10membered lactone (5), and one natural 4-epi-brefeldin C (8), along with seven known compounds were found from isogenesis endophytes Aspergillus fumigatus, Penicillium janthinellum, Nigrospora sp., and Stagonosporopsis sp. induced by host Nicotiana tabacum medium and co-culture. The structures were determined mainly by spectroscopic methods, including extensive 1D, 2D NMR, MS techniques, ECD calculation, and Mosher's method. Compound 2 possessed a novel 1, 3-dioxetane residue and cyclohexane-containing terpenoid skeleton. Compounds 2, 4-7 and 10 showed significant antifungal activities against the plant pathogen Nigrospora sp. with MICs of 1 mu g/mL. 2, 4, 5-7, and 10 indicated antifungal activities against Penicillium janthinellum, Aspergillus fumigatus, Phomopsis sp., and Alternaria sp. with MICs <= 8 mu g/mL. Compounds 2, 6-8, and 10 (50 mu g/cm2) and microbial fermentation extracts (100 mu g/cm2) showed antifeedant activities against silkworms with feeding deterrence indices of 21-100%.

• 单位
  云南大学