Fluorescence spectroscopy based on ZnSe quantum dots to detect berberine

摘要

The bovine serum albumin (BSA)-modified ZnSe quantum dots (QDs) possessed water solubility were applied in determining berberine within water solutions by fluorescence spectroscopy. According to our findings, the mean size of those synthesized QDs was approximately 12 nm, as verified through X-ray diffraction (XRD), nanoparticle size analysis and transmission electron microscopy (TEM). Besides, berberine within physiological buffer solution was used to markedly quench fluorescence intensity for those as-modified ZnSe QDs, with the best value being achieved after 10 min of incubation at 25 degrees C and pH 7.0. At the most suitable conditions, berberine showed the limit of detection (LOD) of 5.27x10(-7) mol . L-1. Additionally, the quenching mechanism was also examined, which was suggested as the static quenching process, as certified via constant quenching rate K-q (3.28x10(13) L . mol(-1) . s(-1)).

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