Summary

Two known polyphenols named apigenin 7-O-β- d -glucopyranoside ( S1 ) and querctine-3-O-glucoside ( S2 ), along with another two new compounds apigenin 4′-geranyl-8-glucopyranosyl-7-O-α-glucopyranoside ( S3 ) and apigenin 4′-pernyl-8-glucopyranosyl -7-O-α-glucopyranoside ( S4 ), were isolated from the leaves of Cupressus sempervirens . Structure elucidation of the isolated polyphenols was established on the basis of detailed spectroscopic analysis like 1D and 2D NMR analyses including 1 H NMR, 13 C NMR, COSY, DEPT, HMQC, UV, and Electron Spray Ionization Mass Spectroscopy (ESI-MS). Density Functional Theory (DFT) of computational, Petra/Osiris/Molinspiration (POM), and docking analyses methods were applied in the structural validation of new isolated compounds. The isolated compounds S1-S4 showed significant cytotoxicity against human hepatocellular liver carcinoma HepG2 cells, MCF-7, HC116 and A549.

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