Immobilization of Kluyveromyces marxianus K21 via coaxial electrospinning of PVA and sugarcane bagasse composite for bioethanol production

Authors：Chen, Hung-Yueh; Khumsupan, Darin; Patel, Anil Kumar; Kee, Phei Er; Ng, Hui-Suan; Hsu, Hsien-Yi; Lin, Shin-Ping; Cheng, Kuan-Chen^*

Source：Applied Energy, 2024, 356: 122405.

DOI：10.1016/j.apenergy.2023.122405

• Kluyveromyces marxianus K21
• Bioethanol
• Sugarcane bagasse
• Coaxial electrospinning
• Microbial immobilization

The study employed coaxial electrospinning technology to design an immobilization system for ethanol pro-duction from a sugarcane bagasse (SCB) and polyvinyl alcohol (PVA) composite. Using response surface methodology (RSM), the most suitable pretreatment conditions were 1.84 N H2SO4 at 70.85 degrees C for 30 min which resulted in 4.73 mg polysaccharide per 1 mL of SCB being extracted, comparing to the theoretical value of 4.51 mg. According to physical characterization analyses, both PVA/SCB and yeast-embedded PVA/SCB composites had superior strength and ductility compared to PVA-only sample. With 36.5 g/L of sugar consumption, a total of 18.01 g/L ethanol was produced after 10 h of cultivation from PVA/SCB/K21 which was higher than that from the freely suspended culture (15.31 g/L at 12 h), resulting in a 15% increase in yield. Furthermore, the PVA/ SCB/K21 system was able to maintain constant ethanol production even after 21 days of storage, and could be reused in fermentation for at least 8 cycles. The findings showed that the PVA/SCB/K21 nanothreads is an ideal system for repeated bioethanol production while upcycling sugarcane bagasse biomass waste.

• Institution
  中国医科大学; 1