Circular RNA circEYA3 promotes the radiation resistance of hepatocellular carcinoma via the IGF2BP2/DTX3L axis

作者：Hu, Pan; Lin, Letao; Huang, Tao; Li, Zhenyu; Xiao, Meigui; Guo, Huanqing; Chen, Guanyu; Liu, Dengyao; Ke, Miaola; Shan, Hongbo^*; Zhang, Fujun^*; Zhang, Yanling^*

来源：Cancer Cell International, 2023, 23(1): 308.

DOI：10.1186/s12935-023-03168-2

• Circular RNA
• RNA-binding protein
• I-125 brachytherapy
• Radiation resistance

Background Hepatocellular carcinoma (HCC) has a high incidence and mortality rate despite various treatment options, including I-125 seed implantation. However, recurrence and radiation resistance remain challenging issues. Hsa_circ_0007895 (circEYA3)-derived from exons 2-6 of EYA3-facilitates the proliferation and progression of pancreatic ductal adenocarcinoma. However, the role of circEYA3 in HCC I-125 radiation resistance remains unclear. Thus, we aimed to investigate the functions and underlying molecular mechanisms of circEYA3 in HCC under I-125 and X-ray irradiation conditions. Methods CircEYA3 was identified by RNA-seq in patients with HCC before and after I-125 seed implantation treatment, followed by fluorescence in situ hybridization and RNase R assays. The radiosensitivity of HCC cell lines irradiated with I-125 seeds or external irradiation were evaluated using the Cell Counting Kit 8, flow cytometry, gamma H2A.X immunofluorescence and comet assays. RNA pull-down and RNA immunoprecipitation assays were performed to explore the interactions between circEYA3 and IGF2BP2. DTX3L mRNA was identified by RNA-seq in PLC/PRF/5 cells with overexpressed circEYA3. The corresponding in vitro results were verified using a mouse xenograft model. Results CircEYA3 decreased the radiosensitivity of HCC cells both in vitro and in vivo. Notably, using a circRNA pulldown assay and RNA-binding protein immunoprecipitation, we identified IGF2BP2 as a novel and robust interacting protein of circEYA3. Mechanistically, circEYA3 binds to IGF2BP2 and enhances its ability to stabilize DTX3L mRNA, thereby specifically alleviating radiation-induced DNA damage in HCC cells. Conclusions Our findings demonstrate that circEYA3 increases the radioresistance of HCC to I-125 seeds and external irradiation via the IGF2BP2/DTX3L axis. Thus, circEYA3 might be a predictive indicator and intervention option for I-125 brachytherapy or external radiotherapy in HCC.

• 单位
  中山大学; 南方医科大学