Cerebrospinal fluid metabolomic and proteomic characterization of neurologic post-acute sequelae of SARS-CoV-2 infection

作者：Chen, Shilan; Liang, Jianhao; Chen, Dingqiang; Huang, Qiyuan; Sun, Kaijian; Zhong, Yuxia; Lin, Baojia; Kong, Jingjing; Sun, Jiaduo; Gong, Chengfang; Wang, Jun; Gao, Ya^*; Zhang, Qingguo^*; Sun, Haitao^*

来源：Brain, Behavior, and Immunity, 2024, 115: 209-222.

DOI：10.1016/j.bbi.2023.10.016

• Neuro-PASC
• SARS-CoV-2
• Cerebrospinal fluid
• COVID-19
• Multi-omics
• Biomarker

The mechanism by which SARS-CoV-2 causes neurological post-acute sequelae of SARS-CoV-2 (neuro-PASC) remains unclear. Herein, we conducted proteomic and metabolomic analyses of cerebrospinal fluid (CSF) samples from 21 neuro-PASC patients, 45 healthy volunteers, and 26 inflammatory neurological diseases patients. Our data showed 69 differentially expressed metabolites and six differentially expressed proteins between neuro-PASC patients and healthy individuals. Elevated sphinganine and ST1A1, sphingolipid metabolism disorder, and attenuated inflammatory responses may contribute to the occurrence of neuro-PASC, whereas decreased levels of 7,8-dihydropterin and activation of steroid hormone biosynthesis may play a role in the repair process. Additionally, a biomarker cohort consisting of sphinganine, 7,8-dihydroneopterin, and ST1A1 was preliminarily demonstrated to have high value in diagnosing neuro-PASC. In summary, our study represents the first attempt to integrate the diagnostic benefits of CSF with the methodological advantages of multi-omics, thereby offering valuable insights into the pathogenesis of neuro-PASC and facilitating the work of neuroscientists in disclosing different neurological dimensions associated with COVID-19.

• 单位
  1; 南方医科大学; y