Electron-Transfer Study and Single Nucleotide Discrimination of a DNA Sequence on a Polymer Gold Electrode (PGE) by Differential Pulse Voltammetry (DPV)

Authors：Xu, Long; Zhou, Binyu; Song, Yaling; Cai, Xu^*; Lu, Wei^*

Source：Analytical Letters, 2022, 55(12): 1919-1931.

DOI：10.1080/00032719.2022.2035390

• Cyclic voltammetry (CV)
• differential pulse voltammetry (DPV)
• DNA biosensing
• electrochemistry
• polymer gold electrode (PGE)

Although the development of DNA sensors, including gold-based electrodes, has made rapid progress, it is essential to characterize DNA-gold surfaces to better understand their operation and improve performance. Recently, a novel polymer gold electrode (PGE) that shows promising performance as DNA biosensor has been designed. Herein, superior electrochemical characteristics of PGE were further studied and reported. For example, a striking difference of potential between Ru(NH3)(6)(3+) adsorbed onto DNA and in solution was observed, indicating the presence of distinct electron transport pathways of the two states of Ru(NH3)(6)(3+). Because of the superior electrochemical properties of the PGE, discrimination of single nucleotide mismatch was achieved without signal amplification or sensitive labeling.

• Institution
  中国医科大学; 哈尔滨医科大学