Magnetic nano fluids for isolation of genomic DNA and total RNA from various prokaryote and eukaryote cells.

作者：Ghahari, Sajjad; Ghahari, Somayeh; Nematzadeh, Ghorban Ali^*

来源：J Chromatogr B Analyt Technol Biomed Life Sci, 2018, 1102-1103: 125-134.

DOI：10.1016/j.jchromb.2018.10.006

• Biocompatibility beads
• Magnetic nano fluid particles
• Nucleic acid isolation
• Purification kit

The correct isolation of nucleic acid from various cells is an important preliminary step before many biochemical and diagnostic processes such as cloning, sequencing, replication, hybridization, and complementary DNA (cDNA) synthesis. In this study, the coated magnetic nanoparticles (MNFs) with Tween 20 and oleic acid because of paramagnetic and bio-compatibility properties used in the extractions of genomic DNA (gDNA) and total RNA from prokaryote and eukaryote cells. The amount and accuracy of gDNA and total RNA extracted were proved via agarose gel electrophoresis, digestion and polymerase chain reaction (PCR) techniques. According to UV-Vis spectrophotometry data and gDNA and ribosomal RNA (rRNA) bands observed on the agarose gel, the results showed that extraction of this nano-kit can be comparable with the existing methods used to purifying nucleic acids such as purification based on the use of Cetyltrimethylammonium bromide (CTAB) and phenol-chloroform methods. Characterization of the particles defines them to be ~34.85nm in diameter and exhibiting high saturation magnetization (28emu/g). Elimination of hazardous reagents such as phenol and chloroform from extraction solutions, the replacement for inorganic coating such as silica with organic oil, and reduction of reaction time are some advantages of this method. Therefore, according to the challenges in the nucleic acid purification pathway, the use of these kits can be remarkable.