Enzymatic depolymerization of plastic materials by a highly efficient two-enzyme system

作者：Sun, Jiaojiao; Pang, Yaxing; Lei, Ziyi; Ouyang, Bo; Lai, Wanxian; Wang, Yonghua^*; Lan, Dongming^*

来源：BIOCHEMICAL ENGINEERING JOURNAL, 2024, 204: 109222.

DOI：10.1016/j.bej.2024.109222

• PET degradation
• Enzyme screening
• Fusion protein
• TPA
• Recycle

Advancing the efficiency of polyethylene terephthalate (PET) degradation is pivotal for sustainable and versatile PET recycling. In this study, we initially screened eight esterases, pinpointing Pyrobaculum calidifontis VA1 esterase (PCEST) as markedly effective in decomposing PET intermediate products. Building on this discovery, we engineered fusion proteins combining a robust cutinase (HRC) with PCEST, connected by flexible glycineserine linkers. We further optimized the linker length, the sequential arrangement and the enzymatic conditions of the conjugated enzymes. Notably, the synergistic activity of the HRC-PCEST fusion protein significantly enhanced the breakdown of PET, thereby promising to streamline recycling processes. Collectively, this methodology not only propels the efficiency of PET degradation but also broadens the horizons for sustainable and versatile PET waste management protocols.

• 单位
  中国科学院