Label-free detection of DNA hybridization using transistors based on CVD grown graphene

作者：Chen T Y; Loan P T K; Hsu C L; Lee Y H; Tse Wei Wang J; Wei K H; Lin C T^*; Li L J

来源：Biosensors and Bioelectronics, 2013, 41(1): 103-109.

DOI：10.1016/j.bios.2012.07.059

• CVD graphene
• DNA sensing
• Gold-transfer method
• Label-free detection
• Liquid-gated transistors

The high transconductance and low noise of graphene-based field-effect transistors based on large-area monolayer graphene produced by chemical vapor deposition are used for label-free electrical detection of DNA hybridization. The gate materials, buffer concentration and surface condition of graphene have been optimized to achieve the DNA detection sensitivity as low as 1pM (10-12M), which is more sensitive than the existing report based on few-layer graphene. The graphene films obtained using conventional PMMA-assisted transfer technique exhibits PMMA residues, which degrade the sensing performance of graphene. We have demonstrated that the sensing performance of the graphene samples prepared by gold-transfer is largely enhanced (by 125).

• 单位
  中国科学院