Loss of RNA-Binding Protein HuR Leads to Defective Ependymal Cells and Hydrocephalus

作者：Han, Xiu; Shen, Xuning; Wang, Min; Wang, Xin; Jian, Youli; Yang, Chonglin; Guo, Weixiang^*

来源：Journal of Neuroscience, 2022, 42(2): 202-219.

DOI：10.1523/JNEUROSCI.1317-21.2021

• ependymal cell
• HuR
• hydrocephalus

Multiciliated ependymal cells line the ventricle wall and generate CSF flow through ciliary beating. Defects in ependymal cells cause hydrocephalus; however, there are still significant gaps in our understanding the molecular, cellular and developmental mechanisms involved in the pathogenesis of hydrocephalus. Here, we demonstrate that specific deletion of RNA-binding protein (RBP) Hu antigen R (HuR) in the mouse brain results in hydrocephalus and causes postnatal death. HuR deficiency leads to impaired ependymal cell development with defective motile ciliogenesis in both female and male mice. Transcriptomewide analysis reveals that HuR binds to mRNA transcripts related to ciliogenesis, including cilia and flagella associated protein 52 (Cfap52), the effector gene of Foxj-1 and Rfx transcriptional factors. HuR deficiency accelerates the degradation of Cfap52 mRNA, while overexpression of Cfap52 is able to promote the development of HuR-deficient ependymal cells. Taken together, our results unravel the important role of HuR in posttranscriptional regulation of ependymal cell development by stabilizing Cfap52 mRNA.

• 单位
  云南大学; 中国科学院研究生院; y