Integration of Isothermal Enzyme-Free Nucleic Acid Circuits for High-Performance Biosensing Applications

作者：Wang, Siyuan; Shang, Jinhua; Zhao, Bingyue; Wang, Huimin^*; Yang, Changying; Liu, Xiaoqing; Wang, Fuan^*

来源：ChemPlusChem, 2023, 88(10).

DOI：10.1002/cplu.202300432

• circuit
• DNAzyme
• hybridization
• self-assembly
• signal amplification

The isothermal enzyme-free nucleic acid amplification method plays an indispensable role in biosensing by virtue of its simple, robust, and highly efficient properties without the assistance of temperature cycling or/and enzymatic biocatalysis. Up to now, enzyme-free nucleic acid amplification has been extensively utilized for biological assays and has achieved the highly sensitive detection of various biological targets, including DNAs, RNAs, small molecules, proteins, and even cells. In this Review, the mechanisms of entropy-driven reaction, hybridization chain reaction, catalytic hairpin assembly and DNAzyme are concisely described and their recent application as biosensors is comprehensively summarized. Furthermore, the current problems and the developments of these DNA circuits are also discussed. @@@ Nucleic acids, universally acknowledged as the essential carriers of genetic information, can be artificially manipulated and assembled into simple, robust, and sensitive biosensors without the assistance of temperature cycling or enzymes. These smart biosensors based on entropy-driven reaction, hybridization chain reaction, catalytic hairpin assembly, and DNAzyme, as well as their extensive applications are summarized in this Review.image

• 单位
  武汉大学