G-rich sequence-specific recognition and scission of human genome by PNA/DNA hybrid G-quadruplex formation

作者：Ishizuka Takumi^*; Yang Jie; Komiyama Makoto; Xu Yan

来源：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 51(29).

DOI：10.1002/anie.201201176

• DNA recognition
• DNA scission
• Double stranded DNA
• G-quadruplexes
• Gene manipulation
• Human genomes
• Peptide nucleic acid
• Site selective
• Target sites

Hole in one: A single peptide nucleic acid (PNA) effectively targets the G-rich region in double-stranded DNA through formation of a PNA/DNA hybrid G-quadruplex. Only one target site in the whole human genome was selectively cleaved by the hybrid G-quadruplex. Such site-selective scission of DNA is central to gene manipulation for molecular biology, biotechnology, and therapy.

• 单位
  6; 1; university of tokyo