Long non-coding RNA SNHG29 regulates cell senescence via p53/p21 signaling in spontaneous preterm birth

作者：Jiang, Jiayi; Hu, Haoyue; Chen, Qian; Zhang, Yi; Chen, Wenqian; Huang, Qiuyu; Chen, Xiaoli; Li, Jing^*; Zhong, Mei^*

来源：Placenta, 2021, 103: 64-71.

DOI：10.1016/j.placenta.2020.10.009

• SNHG29
• Spontaneous preterm birth
• Cellular senescence
• Oxidative stress
• p53/p21
• SASP

Introduction: Spontaneous preterm birth affects>5-18% of pregnancies and causes infant morbidity and mortality. Long non-coding RNAs can regulate gene expression and have been associated with preterm birth. In this study, we investigated whether the long non-coding RNA SNHG29 was associated with spontaneous preterm birth. @@@ Methods: We collected the placentas from women who underwent preterm/full-term birth with/without labor. We determined the levels of expression of SNHG29 in the placental tissues using quantitative real-time polymerase chain reaction. We generated a senescence model by treating HTR8/SVneo cells with 200 mu M H2O2 for 2 h. The degree of senescence induced in cells depleted of or overexpressing SNHG29 was determined by measuring senescence-associated gene expression and beta-galactosidase activity. @@@ Results: SNHG29 was overexpressed in the placentas of women who delivered preterm with labor and in HTR8/SVneo cells treated with H2O2 (p < 0.05). The levels of mRNA of p53 and p21, protein levels of p53, phosphop53, p2land phospho-p21, and beta-galactosidase activity was decreased in HTR8/SVneo cells depleted of SNHG29, while the opposite trend was observed in HTR8/SVneo cells overexpressing SNHG29 (p < 0.05). We observed an increase in the expression of IL-8 and TNF-alpha in senescent HTR8/SVneo cells (p < 0.05). @@@ Discussion: SNHG29 was overexpressed in placentas from women who delivered preterm with labor compared to those in women who underwent preterm birth without labor and full-term birth with/without labor. High levels of SNHG29 enhanced senescence in vivo. The increase in pro-inflammatory cytokine expression and release by senescent cells may be pivotal to the pathophysiology of spontaneous preterm birth.

• 单位
  南方医科大学