A fluorescence-based analysis of aristolochic acid-derived DNA adducts

作者：Romanov V^*; Sidorenko V; Rosenquist T A; Whyard T; Grollman A P

来源：ANALYTICAL BIOCHEMISTRY, 2012, 427(1): 49-51.

DOI：10.1016/j.ab.2012.03.027

• Aristolochic acids
• DNA adducts
• Fluorescence detection

Aristolochic acids (AAs), major components of plant extracts from Aristolochia species, form (after metabolic activation) pro-mutagenic DNA adducts in renal tissue. The DNA adducts can be used as biomarkers for studies of AA toxicity. Identification of these adducts is a complicated and time-consuming procedure. We present here a fast, nonisotopic, fluorescence-based assay for the detection of AA-DNA adducts in multiple samples. This approach allows analysis of AA adducts in synthetic DNA with known nucleotide composition and analysis of DNA adducts formed from chemically diverse AAs in vitro. The method can be applied to compare AA-DNA adduct formation in cells and tissues.

• 单位
  State University of New York at Stony Brook