OCT4 expression transactivated by GATA protein is essential for non-rodent trophectoderm early development

作者：Bou, Gerelchimeg; Guo, Jia; Liu, Shichao; Guo, Shimeng; Davaakhuu, Gantulga; Lv, Qi; Xue, Binghua; Qiao, Shupei; Lv, Jiawei; Weng, Xiaogang; Zhao, Jianchao; Zhang, Yuting; He, Yilong; Zhang, Heng; Chai, Zhuang; Liu, Yan; Yu, Yanbo; Qu, Bo; Sun, Ruizhen; Shen, Xinghui; Lei, Lei; Liu, Zhonghua^*

来源：Cell Reports, 2022, 41(8): 111644.

DOI：10.1016/j.celrep.2022.111644

• INNER CELL MASS
• EARLY LINEAGE SEGREGATION
• MOUSE EMBRYOS
• PREIMPLANTATION EMBRYOS
• PRIMITIVE ENDODERM
• MICE LACKING
• CDX2
• PLURIPOTENCY
• SOX2
• BOVINE

Oct4 is exclusively expressed in rodent inner cell mass (ICM) but silenced in its trophectoderm (TE). However, for many non-rodent animals, including pig, cattle, rabbit, goat, and human, OCT4 has a remarkable expres-sion in early TE. This study, applying pig as the main research model, proves that OCT4 expression in TE is supported by a unique GATA motif in the OCT4 upstream conserved regulatory region, and GATA4 is respon-sible for its activation. Moreover, OCT4 acts as a specific regulator of a narrow range of genes (including BCL2A1 and HNRNP2AB1) that are essential for the first wave of rapid proliferation in early TE. This study describes the regulatory mechanism to direct the OCT4 expression and its significance in TE of porcine pre -implantation embryo.

• 单位
  哈尔滨医科大学; 东北农业大学