Altered composition of the oral microbiome in integrin beta 6-deficient mouse

作者：Uehara, Osamu; Bi, Jiarui; Zhuang, Deshu; Koivisto, Leeni; Abiko, Yoshihiro; Hakkinen, Lari; Larjava, Hannu^*

来源：Journal of Oral Microbiology, 2022, 14(1).

DOI：10.1080/20002297.2022.2122283

• B6 integrin-null mouse
• oral microbiome
• 16S ribosomal RNA
• QIIME 2

In periodontal disease (PD), bacterial biofilms suppress beta 6 integrin expression transforming growth factor-beta 1 signaling, resulting in gingival inflammation and bone loss. beta 6 integrin-null (Itgb6(-/)(-)) mice develop spontaneous PD. The aim of this study was to unravel potential differences in oral microbiome in wild-type (WT) and Itgb6(-/-) FVB mice. Mouse oral micro-biome was analyzed from 3- and 6-month-old WT and Itgb6t(-/-). The periodontal inflammation and spontaneous bone loss were present in 3-month-old and advanced in 6-month-old Itgb6(-/-) mice. The observed amplicon sequence variants (ASVs) of alpha diversity showed close similarity in 3-month-old and 6-month-old Itgb6(-/-) mice. Chao1 and ACE methods revealed that the microbiome in Itgb6(-/-) mice showed less diversity compared to the WT. UniFrac Principal Coordinate analyses (PCoA) showed a clear spatial segregation and clustering between Itgb6(-/-) and WT mice in general, and between 3-month- and 6-month-old WT mice. Weighted PCoA showed the tight clustering and distinct separation of individual mouse samples within Itgb6(-/-) and WT. The most abundant microbial classes varied between the sample groups. However, the genus Aggregatibacter significantly increased in the 6-month-old Itgb6(-/- )mice. beta 6 integrin-deficient mice develop periodontal inflammation that may relate to dysbiosis in the microbiome that further promotes the disease process.

• 单位
  1; 6; 哈尔滨医科大学