Vinculin Identified as a Potential Biomarker in Hand-Arm Vibration Syndrome Based on iTRAQ and LC-MS/MS-Based Proteomic Analysis

摘要

Local vibration can induce vascular injuries, one exampleis thehand-arm vibration syndrome (HAVS) caused by hand-transmitted vibration(HTV). Little is known about the molecular mechanism of HAVS-inducedvascular injuries. Herein, the iTRAQ (isobaric tags for relative andabsolute quantitation) followed by liquid chromatography-tandemmass spectrometry (LC-MS/MS) proteomics approach was appliedto conduct the quantitative proteomic analysis of plasma from specimenswith HTV exposure or HAVS diagnosis. Overall, 726 proteins were identifiedin iTRAQ. 37 proteins upregulated and 43 downregulated in HAVS. Moreover,37 upregulated and 40 downregulated when comparing severe HAVS andmild HAVS. Among them, Vinculin (VCL) was found to be downregulatedin the whole process of HAVS. The concentration of vinculin was furtherverified by ELISA, and the results suggested that the proteomics datawas reliable. Bioinformative analyses were used, and those proteinsmainly engaged in specific biological processes like binding, focaladhesion, and integrins. The potential of vinculin application inHAVS diagnosis was validated by the receiver operating characteristiccurve.

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