In vitro PROPAGATION OF Liparis nervosa (Thunb.) Lindl., AN ENDANGERED MEDICINAL ORCHID

作者：Ren, Yan; Gao, Jin-Rong; Cai, Shou-Meng; Yan, Hong-Mei; Huang, Heng-Yu^*

来源：Acta Scientiarum Polonorum-Hortorum Cultus, 2023, 22(5): 73-87.

DOI：10.24326/asphc.2023.5023

• Key words: in vitro regeneration
• proliferation method
• basal stem cluster bud
• tiller bud
• protocorm-like
• body
• Liparis nervosa (Thunb.) Lindl.

In vitro regeneration was studied to protect the rare Chinese medicinal orchid Liparis nervosa (Thunb.) Lindl. The mixtures of protocorm and seeding and the stem tip were used as explants. The results revealed that the best essential medium for L. nervosa growth was 1/3 MS medium with 25 g <middle dot> L-1 sucrose, 50 g <middle dot> L-1 banana puree, 40 g <middle dot> L-1 mashed potato, and 1.0 g <middle dot> L-1 AC (MS1); MS1 medium with 0.5 mg <middle dot> L-1 BA, 0.05 mg <middle dot> L-1 2,4-D, and 1.5 mg <middle dot> L-1 NAA was optimal for proliferation. When stem tips were cultured in a proliferation medium, four types of proliferation occurred: basal stem cluster bud (occurring at the basal node), tiller bud (occurring at the root), protocorm-like body (occurring at the plant's base incision), and high-position bud (occurring on plant stem nodes other than the basal nodes). Four methods produced 10.12 proliferation co -efficients. In the MS1 medium with 0.5 mg <middle dot> L-1 NAA, the plantlets rooted 100%, and the rooted plantlets survived 100% after domestication and transplantation.

• 单位
  云南大学