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摘要
Objective To observe the effect of Hedysarum Polybotrys Polysacchcaide(HPS) on the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) and glucose transporter-4 (GLuT4) in myocardial tissue in diabetes (db/db)mice with diabetic cardiomyopathy.Methods The male db/db mice of 6-weeks age were randomly divided into 5 groups on the basis of weight:model group(0.9％ NaC1 by gavage),control group(4 mg · kg-1 · d-1 rosiglitazone) and high,middle and low doses(200,100,and 50 mg · kg-1 · d-1HPS) experimental groups.The same age were included in the normal group.Continuously administrating for 8 weeks.The blood glucose of mice was tested before and after administration of 2,4,6,8 weeks later.The activity of blood lipid,superoxide dismutase (SOD),glutathione peroxidase (GSH-PX) and the content of malondialdehyde (MDA) were detected by biochemical method after 8 weeks.Reverse transcription polymerase chain reaction method and Western blotting method were used to detect the expression of PPARγ,GluT4 mRNA and protein in myocardial tissue.Results After the treatment of HPS for 8 weeks,compared to the model group on the blood glucose (BG) was (23.17 ± 2.55)mmol · L-1,on the triglyceride (TG) was (5.78 ±0.50) mmol · L-1and on the total cholesterol (TC) was (5.93 ±0.60) mmol · L-1;the BG in high and middle doses experimental groups and control group were (16.49 ± 3.64),(17.80 ± 4.40),(16.76 ± 3.25),mmol · L-1,the TG in above groups were (1.59 ±0.43),(4.02 ±0.54),(1.12 ±0.32) mmol · L-1;the TC in above groups were (2.77 ± 0.40),(4.65 ± 0.58),(4.85 ± 0.48) mmol · L-1 with statistical difference (all P ＜ 0.01).Compared to the model group on the SOD was (140.70 ± 1.04)mg · mL-1,on the GSH-PX was (110.91 ±0.82) U · mg-1,on the MDA was(145.70 ± 1.10) mg · mL-1,the SOD in three doses experimental groups and control group were (145.81 ± 0.99) (142.21 ± 1.09),(145.70 ± 1.10) mg-1,-· mL respectively,and the GSH-PX in that three doses groups were (114.94 ± 0.78),(112.10 ± 0.86),(114.84 ± 0.86) U · mg-1,and the MDA were (5.82 ± 0.52),(6.62 ± 0.67),(5.80 ± 0.52) nmol · mg-1 with statistical difference (all P ＜ 0.05 or P ＜ 0.01).Compared to the model group on the mRNA and protein of PPAR-γwere 0.34 ±0.11,0.75 ±0.12;on the mRNA and protein of GluT-4 in model group were 0.26 ± 0.01,0.42 ± 0.02,but the expression of mRNA and protein of PPAR-γ in high,middle and low doses experimental groups and control group were 0.76 ± 0.06,0.56 ± 0.08,0.45 ± 0.08,0.79 ± 0.10;1.78 ± 0.08,1.44 ± 0.07,1.07 ± 0.05,1.63 ± 0.02 with increased significantly (all P＜0.01).The expression of mRNA and protein of GluT-4 in the four groups were 0.68 ± 0.05,0.48±0.03,0.49 ±0.03,0.93 ±0.05;0.61 ±0.01,0.45 ±0.02,0.42 ±0.01,0.59 ±0.01 with increased significantly also(all P ＜ 0.O1),especially in the high dose of HPS.Conclusion HPS might improve the blood glucose and blood lipid metabolism disorders of diabetic db/db mice.HPS reducing the blood glucose and blood lipids and raising the expression of PPAR-γand GluT-4.HPS slow down the progress of diabetic cardiomyopathy.
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单位甘肃中医学院
