Delineating charge and capacitance transduction in system-integrated graphene-based BioFETs used as aptasensors for malaria detection

Authors：Figueroa-Miranda, Gabriela; Liang, Yuanying; Suranglikar, Mohit; Stadler, Matthias; Samane, Nagesh; Tintelott, Marcel; Lo, Young; Tanner, Julian A.; Vu, Xuan T.; Knoch, Joachim; Ingebrandt, Sven; Offenhaeusser, Andreas; Pachauri, Vivek^*; Mayer, Dirk^*

Source：Biosensors and Bioelectronics, 2022, 208: 114219.

DOI：10.1016/j.bios.2022.114219

• Malaria detection
• Plasmodium falciparum
• Graphene field-effect transistor
• Aptasensor
• Aptamer-protein interaction
• Label-free detection

Despite significant eradication efforts, malaria remains a persistent infectious disease with high mortality due to the lack of efficient point-of-care (PoC) screening solutions required to manage low-density asymptomatic parasitemia. In response, we demonstrate a quantitative electrical biosensor based on system-integrated twodimensional field-effect transistors (2DBioFETs) of reduced graphene oxide (rGO) as transducer for high sensitivity screening of the main malaria biomarker, Plasmodium falciparum lactate dehydrogenase (PfLDH). The 2DBioFETs were biofunctionalized with pyrene-modified 2008s aptamers as specific PfLDH receptors. While we systematically optimize biosensor interface for optimal performance, aptamer-protein transduction at 2DBioFETs is elucidated based on delineation of charge and capacitance in an updated analytical model for two-dimensional rGO/biofunctional layer/electrolyte (2DiBLE) interfaces. Our 2DBioFET-aptasensors display a limit-of-detection down to 0.78 fM (0.11 pg/mL), dynamic ranges over 9 orders of magnitude (subfemto to submicromolar), high sensitivity, and selectivity in human serum validating their diagnostic potential as rapid PoC tests for malarial management.

• Institution
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