Low-Cost Full-Range Detection of C-Reactive Protein in Clinical Samples by Aptamer Hairpin Probes and Coprecipitation of Silver Ions and Gold Nanoparticles

作者：Yan, Hong; Hu, Xiumei; Shao, Huaze; Li, Jincheng; Deng, Jieqi; Liu, Lihong^*

来源：Analytical Chemistry, 2023, 95(32): 11918-11925.

DOI：10.1021/acs.analchem.3c01131

• QUANTIFICATION
• IMMUNOASSAY
• SANDWICH
• RISK

C-reactiveprotein (CRP) levels can vary widely related to diversedisease contexts. However, expensive antibodies have impeded the clinicalutility of antibody-based full-range CRP assays, especially in developingcountries. Herein, we established a low-cost, antibody-free, 96-wellplate-based full-range CRP detection method by combining gold nanoparticles(AuNPs), silver iodide (AgI), Eosin Y, and the aptamer hairpin probe(AHP) with Ag+-mediated cytosine-cytosine mismatches,that is, the Au@AgI/Eosin Y-AHP method. After binding the target CRP,the AHP released Ag+, which subsequently induced the aggregationof AuNPs on the surface of AgI colloids, resulting in a significantincrease in the adsorption of Eosin Y on the surface of AuNPs. Thechanges in fluorescence intensity (FI) of Eosin Y in the supernatewithout and with CRP were proportional to the concentration of theCRP in the wide range of 0.01-40 ng/mL (r =0.9969), and 96 samples can be detected in 96-well plates simultaneouslyby a microplate reader within 45 min. Remarkably, the CRP levels of100 clinical samples achieved with the Au@AgI/Eosin Y-AHP had a goodcorrelation with those obtained with the latex-enhanced immune turbidimetryassay (r = 0.986). Furthermore, the kit based onthe Au@AgI/Eosin Y-AHP method costs only $8.1 for 100 tests. Therefore,the new method is beneficial for less developed areas where expensiveassays are not affordable.

• 单位
  南方医科大学