An activity-based fluorescent probe and its application for differentiating alkaline phosphatase activity in different cell lines

作者：He, Yong; Yu, Junli; Hu, Xiangzi; Huang, Shumei; Cai, Lili; Yang, Liu; Zhang, Huatang^*; Jiang, Yin^*; Jia, Yongguang; Sun, Hongyan^*

来源：Chemical Communications, 2020, 56(87): 13323-13326.

DOI：10.1039/d0cc06129h

Herein, a new fluorescent probe, AE-Phos, is reported for detecting the ALP activity with the combined advantages of aggregation-induced emission (AIE) and excited state intramolecular proton transfer (ESIPT). Further detailed fluorescence experiments demonstrated that AE-Phos exhibited excellent selectivity and sensitivity, a large Stokes shift, and a fast response towards ALP. Furthermore, AE-Phos was applied to imaging the ALP activity in different cell lines quantitatively.

• 单位
  6; 广东工业大学; y; 中山大学