Genome-wide identification and expression analysis of anthocyanin synthesis-related R2R3-MYB gene family in purple passion fruit (Passiflora edulis)

Authors：Ni, Ying; Cui, Zeyang; Zhang, Zhiting; Chen, Huiying^*; Zhou, Sheng

Source：Materials Express, 2023, 13(3): 467-481.

DOI：10.1166/mex.2023.2366

• Passion Fruit (Passiflora edulis)
• MYB Transcription Factors
• Anthocyanin Biosynthesis
• Bioinformatics Analysis
• Gene Expression and Regulation
• Gene Structure

IP: 203 8 109 20 On: Tue 13 Jun 2023 07:08:30 The myeloblastosis (MYB) transcription facors (TFs) participate in a varity of physiological and biochemical Copyright: American Scientific Publishers processes, especially in the anthocyanin biosynthsis in plants. However, the comprehensive functional study Delivered by Ingenta of MYB TFs, especially in regulating anthocyanin accumulation in passion fruit (Passiflora edulis) remains limited. To gain a better understanding of the evolutionary history of this family, we conducted a genome-wide analysis of MYB TFs in passion fruit, including phylogenetic analysis, gene structure, conserved motifs, chro-mosomal locations, and collinearity analysis. A total of 254 MYB genes were identified in the passion fruit genome, comprising 128 1R-MYBs, 119 R2R3-MYBs, 5 3R-MYBs and 2 4R-MYBs. Based on the classifica-tion of Arabidopsis thaliana MYB (AtMYB) genes, the passion fruit R2R3-MYB (PeMYB) genes were further divided into 37 subgroups, which were dispersed in 9 chromosomes. The analyses of gene structure and protein conserved motif analyses further supports the clustering results of the phylogenetic tree. RT-qPCR validation revealed that the relative expression of PeMYB67 and PeMYB86 increased during fruit develop-ment, while PeMYB95 exhibited no significant changes. The expression levels of other eight MYB genes involving PeMYB4, PeMYB13, PeMYB32, PeMYB40, PeMYB78, PeMYB115, PeMYB25 and PeMYB118 man-ifested declining regulation alongside fruit ripening. They were either positively or negatively correlated with the accumulation of anthocyanins during fruit growth. Overall, this study provides a comprehensive overview of the passion fruit MYB superfamily genes and lays a foundation for future cloning and functional analysis of PeMYB genes related to anthocyanin accumulation in passion fruit.

• Institution
  桂林理工大学; 仲恺农业工程学院; 肇庆学院