The catalytic properties of DNA G-quadruplexes rely on their structural integrity

作者：Chen, Jielin; Cheng, Mingpan; Wang, Jiawei; Qiu, Dehui; Monchaud, David; Mergny, Jean-Louis; Ju, Huangxian^*; Zhou, Jun^*

来源：CHINESE JOURNAL OF CATALYSIS, 2021, 42(7): 1102-1107.

DOI：10.1016/S1872-2067(20)63744-5

• G-quadruplex
• G-quadruplex DNAzyme
• G-vacancy
• G-quartet integrity
• Guanine surrogate

The influence of the G-quartet structural integrity on the catalytic activity of the G-quadruplex (G4) was investigated by comparing the G4-DNAzyme performances of a series of G4s with a G-vacancy site and a G-triplex (G-tri). The results presented herein not only confirm that the structural integrity of the 3'-end G-quartet is necessary for G4s to be catalytically competent but also show how to remediate G-vacancy-mediated catalytic activity losses via the addition of guanine surrogates in an approach referred to as G-vacancy complementation strategy that is applicable to parallel G4s only. Furthermore, this study demonstrates that the terminal G-quartet could act as a proximal coordinating group and cooperate with the flanking nucleotide to activate the hemin cofactor.

• 单位
  南京大学