Low IL10 serum levels as key factor for predicting the sustained virological response to IFN alpha/ribavirin in Brazilian patients with HCV carrying IL28B CT/TT genotype

作者：Silva Vasconcelos Luydson Richardson; Moura Patricia; do Carmo Rodrigo Feliciano; Pereira Luciano Beltrao; de Mendonca Cavalcanti Maria do Socorro; Barbosa Lins Aroucha Dayse Celia; Dutra Rosa Amalia; Moreira Beltrao Pereira Leila Maria

来源：Human Immunology, 2014, 75(8): 895-900.

DOI：10.1016/j.humimm.2014.06.017

• IL28b
• IL10
• HCV
• Therapy
• Polymorphism

Propose: IL28B polymorphisms rs12979860 CC genotype was associated to protection of HCV infection and sustained virological response (SVR) in HCV infected patients treated with pegIFN alpha/ribavirin (IFN alpha/RIB), however, this polymorphism frequency varies depending on genetic components. Studies with larger number of Brazilian individuals, determining IL28B polymorphisms is lacking. Regarding to treatment response, the levels of IL10 seem to influence response to IFN alpha/RIB therapy. Thus, the IL28B polymorphism frequency was investigated in health controls and infected HCV patients, as well as, in patients who reach SVR vs Non-SVR. Also, to gain insight into the interplay between IL28B genotypes, IL10 levels and therapy response, a subgroup of genotyped HCV patients SVR and Non-SVR were analyzed regarding the IL10 production.
Methods: It was enrolled 487 HCV infected patients and 234 healthy individuals. Patients with response to IFNa/RIB were classified as SVR (n = 81) and Non-SVR (n = 123). TAQMAN probes were used for genotyping the SNP rs12979860, resulting in CC, CT or TT genotypes. In one hundred one patients, the levels IL10 were measured at week 4 of IFN alpha/RIB.
Results: CC genotype was associated to SVR (p = 0.029) and its frequency was higher in healthy individuals vs patients (p = 0.02). Patients carrying CT/TT with IL10 < 10 pg/mL, had a chance of 2.72 to achieve SVR in multivariate model (p = 0.043).
Conclusion: CC genotype was associated to SVR and protection to HCV infection. Moreover, IL28B genotyping and IL10 serum levels could be further explored as a useful algorithm for identify the CF/TT SVR patients.