Human Mesenchymal Stem Cells Elevate CD4(+)CD25(+)CD127(low/-) Regulatory T Cells of Asthmatic Patients via Heme Oxygenase-1

摘要

Up-regulation of CD4(+)CD25(+)CD127(low/-) regulatory T cells (Tregs) is a new target in the treatment of asthma. Human bone marrow mesenchymal stem cells can up-regulate CD4(+)CD25(+)CD127(low/-) regulatory T cells in vitro, meanwhile, heme oxygenase-1 (HO-1) plays an important role in the development and maintenance of CD4(+)CD25(+) regulatory T cells. However the mechanism has not yet been adequately understood. Hence, we wondered what effect of Heme Oxygenase-1 made on regulation of CD4(+)CD25(+)CD127(low/-) regulatory T cells mediated by mesenchymal stem cells. <br>Peripheral blood mononuclear cells isolated from asthmatic patients and healthy controls were co-cultured with human bone marrow mesenchymal stem cells which were pretreated with Hemin (the revulsive of Heme Oxygenase-1), Protoporphyrin. zinc (the inhibitor of Heme Oxygenase-1) and saline. <br>The expression of Heme Oxygenase-1 in MSCs was enhanced by Hemin and inhibited by Protoporphyrin zinc in vitro. Overexpression of Heme Oxygenase-1 elevated the proportion of CD4(+)CD25(+)CD127(low/-) regulatory T cells in CD4(+) T cells, meanwhile, inhibition of Heme Oxygenase-1 decreased the proportion of CD4(+)CD25(+)CD127(low/-) regulatory T cells in CD4(+) T cells as compared with mesenchymal stem cells alone. <br>Taken together, these data demonstrated that Heme Oxygenase-1 contributed to the up-regulation of CD4(+)CD25(+)CD127(low/-) regulatory T cells mediated by mesenchymal stem cells in asthma.

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