Human Mesenchymal Stem Cells Elevate CD4(+)CD25(+)CD127(low/-) Regulatory T Cells of Asthmatic Patients via Heme Oxygenase-1

作者：Li Jian guo; Zhuan sun Yong xun^*; Wen Bing; Wu Hao; Huang Feng ting; Ghimire Hridaya Bibhu; Ran Pi xin

来源：Iranian Journal of Allergy, Asthma and Immunology, 2013, 12(3): 228-235.

• Asthma
• Heme oxygenase-1
• Mesenchymal stromal cells
• T-Lymphocytes, Regulatory

Up-regulation of CD4(+)CD25(+)CD127(low/-) regulatory T cells (Tregs) is a new target in the treatment of asthma. Human bone marrow mesenchymal stem cells can up-regulate CD4(+)CD25(+)CD127(low/-) regulatory T cells in vitro, meanwhile, heme oxygenase-1 (HO-1) plays an important role in the development and maintenance of CD4(+)CD25(+) regulatory T cells. However the mechanism has not yet been adequately understood. Hence, we wondered what effect of Heme Oxygenase-1 made on regulation of CD4(+)CD25(+)CD127(low/-) regulatory T cells mediated by mesenchymal stem cells.
Peripheral blood mononuclear cells isolated from asthmatic patients and healthy controls were co-cultured with human bone marrow mesenchymal stem cells which were pretreated with Hemin (the revulsive of Heme Oxygenase-1), Protoporphyrin. zinc (the inhibitor of Heme Oxygenase-1) and saline.
The expression of Heme Oxygenase-1 in MSCs was enhanced by Hemin and inhibited by Protoporphyrin zinc in vitro. Overexpression of Heme Oxygenase-1 elevated the proportion of CD4(+)CD25(+)CD127(low/-) regulatory T cells in CD4(+) T cells, meanwhile, inhibition of Heme Oxygenase-1 decreased the proportion of CD4(+)CD25(+)CD127(low/-) regulatory T cells in CD4(+) T cells as compared with mesenchymal stem cells alone.
Taken together, these data demonstrated that Heme Oxygenase-1 contributed to the up-regulation of CD4(+)CD25(+)CD127(low/-) regulatory T cells mediated by mesenchymal stem cells in asthma.

• 单位
  1; 广州医学院; 中山大学