Control cell migration by engineering integrin ligand assembly

作者：Hu, Xunwu; Roy, Sona Rani; Jin, Chengzhi; Li, Guanying; Zhang, Qizheng; Asano, Natsuko; Asahina, Shunsuke; Kajiwara, Tomoko; Takahara, Atsushi; Feng, Bolu; Aoki, Kazuhiro; Xu, Chenjie; Zhang, Ye^*

来源：Nature Communications, 2022, 13(1): 5002.

DOI：10.1038/s41467-022-32686-2

• TOP-DOWN
• ADHESION
• RGD
• ORGANIZATION
• CONVERGENCE
• STABILITY
• DYNAMICS
• MEDIATE
• FAK

Engineering peptide assembly that controls integrin ligand presentation on the molecular level possesses by far the highest ligand density, expanding the perspective of ligand-density-dependent modulation. @@@ Advances in mechanistic understanding of integrin-mediated adhesion highlight the importance of precise control of ligand presentation in directing cell migration. Top-down nanopatterning limited the spatial presentation to sub-micron placing restrictions on both fundamental study and biomedical applications. To break the constraint, here we propose a bottom-up nanofabrication strategy to enhance the spatial resolution to the molecular level using simple formulation that is applicable as treatment agent. Via self-assembly and co-assembly, precise control of ligand presentation is succeeded by varying the proportions of assembling ligand and nonfunctional peptide. Assembled nanofilaments fulfill multi-functions exerting enhancement to suppression effect on cell migration with tunable amplitudes. Self-assembled nanofilaments possessing by far the highest ligand density prevent integrin/actin disassembly at cell rear, which expands the perspective of ligand-density-dependent-modulation, revealing valuable inputs to therapeutic innovations in tumor metastasis.

• 单位
  广州医学院; 中国科学院; 西安交通大学; 5; 河北医科大学; y