Naked Eye DNA detection: Synthesis, characterization and DNA binding studies of a novel azo-guanidine

作者：Jamil M; Altaf A A^*; Badshah A; Shafiqullah Ahmad I; Zubair M; Kemal S; Ali M I

来源：Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy , 2013, 105: 165-170.

DOI：10.1016/j.saa.2012.12.020

• Azo-guanidine
• DNA binding
• DNA detection
• Naked Eye DNA detection

A novel class of azo-guanidine compounds is introduced in this article. The novel compound 2-(2-hydroxyphenyl)-1-(phenylamino)-3-(phenylimino)guanidine (AG) was synthesized and well characterized by using different analytical instrumental techniques like elemental analysis, FTIR, 1H and 13C NMR, UV-Visible spectroscopy and cyclic voltammetry. The new compound was found interacting with DNA and shows clear color change in the solution. The AG-DNA complex was qualitatively and quantitatively characterized with UV-Visible spectroscopy and cyclic voltammetry. Electrostatic mode of interaction, clear color change and moderate binding constant (Kb = 104) indicate its potential use as DNA staining agent.