Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings.

作者：Bolsunovsky, A Ya^*; Dementyev, D V; Frolova, T S; Trofimova, E A; Iniatkina, E M; Vasilyev, S A; Sinitsyna, O I

来源：Doklady Biochemistry and Biophysics, 2019, 489(1): 362-366.

DOI：10.1134/S1607672919060024

The effect of gamma-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (</= 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose gamma-radiation on Allium cepa seedlings.