A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea

作者：Han, Fengqing; Yuan, Kaiwen; Sun, Wenru; Zhang, Xiaoli; Liu, Xing; Zhao, Xinyu; Yang, Limei; Wang, Yong; Ji, Jialei; Liu, Yumei; Li, Zhansheng; Zhang, Jinzhe; Zhang, Chunzhi; Huang, Sanwen; Zhang, Yangyong^*; Fang, Zhiyuan^*; Lv, Honghao^*

来源：Nature Communications, 2023, 14(1): 6212.

DOI：10.1038/s41467-023-41916-0

• AGROBACTERIUM-MEDIATED TRANSFORMATION
• POLLEN DEVELOPMENT
• GENE-EXPRESSION
• ARABIDOPSIS
• ETHYLENE
• PROTEIN
• MS1

Male sterility has been used for crop hybrid breeding for a long time. It has contributed greatly to crop yield increase. However, the genetic basis of male sterility has not been fully elucidated. Here, we report map-based cloning of the cabbage (Brassica oleracea) dominant male-sterile gene Ms-cd1 and reveal that it encodes a PHD-finger motif transcription factor. A natural allele Ms-cd1(P Delta-597), resulting from a 1-bp deletion in the promoter, confers dominant genic male sterility (DGMS), whereas loss-of-function ms-cd1 mutant shows recessive male sterility. We also show that the ethylene response factor BoERF1L represses the expression of Ms-cd1 by directly binding to its promoter; however, the 1-bp deletion in Ms-cd1(P Delta-597) affects the binding. Furthermore, ectopic expression of Ms-cd1(P Delta-597) confers DGMS in both dicotyledonous and monocotyledonous plant species. We thus propose that the DGMS system could be useful for breeding hybrids of multiple crop species.

• 单位
  中国科学院; 湖南农业大学