Coupled Lipidomics and Digital Pathology as an Effective Strategy to Identify Novel Adverse Outcome Pathways in Eisenia fetida Exposed to MoS2 Nanosheets and Ionic Mo

作者：Sun, Kailun; White, Jason C.; Qiu, Hao^*; van Gestel, Cornelis A. M.; Peijnenburg, Willie J. G. M.; He, Erkai^*

来源：Environmental Science and Technology, 2023, 57(30): 11009-11021.

DOI：10.1021/acs.est.3c02518

• MoS2 nanosheets
• ultraviolet irradiation
• ecological risk
• liposomes
• coelomocyte
• multi-omics
• molecular mechanisms

Molybdenum disulfide (MoS2) nanosheets areincreasinglyapplied in several fields, but effective and accurate strategies tofully characterize potential risks to soil ecosystems are lacking.We introduce a coelomocyte-based in vivo exposurestrategy to identify novel adverse outcome pathways (AOPs) and molecularendpoints from nontransformed (NTMoS2) and ultraviolet-transformed(UTMoS2) MoS2 nanosheets (10 and 100 mg Mo/L)on the earthworm Eisenia fetida usingnontargeted lipidomics integrated with transcriptomics. Machine learning-baseddigital pathology analysis coupled with phenotypic monitoring wasfurther used to establish the correlation between lipid profilingand whole organism effects. As an ionic control, Na2MoO4 exposure significantly reduced (61.2-79.5%) the cellularcontents of membrane-associated lipids (glycerophospholipids) in earthwormcoelomocytes. Downregulation of the unsaturated fatty acid synthesispathway and leakage of lactate dehydrogenase (LDH) verified the Na2MoO4-induced membrane stress. Compared to conventionalmolybdate, NTMoS2 inhibited genes related to transmembranetransport and caused the differential upregulation of phospholipidcontent. Unlike NTMoS2, UTMoS2 specificallyupregulated the glyceride metabolism (10.3-179%) and lipidperoxidation degree (50.4-69.4%). Consequently, lipolytic pathwayswere activated to compensate for the potential energy deprivation.With pathology image quantification, we report that UTMoS2 caused more severe epithelial damage and intestinal steatosis thanNTMoS(2), which is attributed to the edge effect and higherMo release upon UV irradiation. Our results reveal differential AOPsinvolving soil sentinel organisms exposed to different Mo forms, demonstratingthe potential of liposome analysis to identify novel AOPs and furthermoreaccurate soil risk assessment strategies for emerging contaminants. @@@ A risk assessment framework integratingliposome analysisand multidimensional phenotypic monitoring can effectively identifynovel adverse outcome pathways of nontransformed and ultraviolet-transformedMoS(2) nanosheets to soil sentinel species Eisenia fetida.

• 单位
  上海交通大学