A Ganoderma lucidum polysaccharide F31 alleviates hyperglycemia through kidney protection and adipocyte apoptosis

作者：Jiao, Jiaqi; Yong, Tianqiao; Huang, Longhua; Chen, Shaodan; Xiao, Chun^*; Wu, Qingping^*; Hu, Huiping; Xie, Yizhen; Li, Xiangmin; Liu, Yuancao; Cai, Manjun; Zhang, Ruifang; Xie, Ting; Liu, Rongjie

来源：International Journal of Biological Macromolecules, 2023, 226: 1178-1191.

DOI：10.1016/j.ijbiomac.2022.11.231

• Hypoglycemia
• Kidney protection
• Adipocyte apoptosis
• Ganaderma lucidum
• Polysaccharides
• Metabolomics

In this paper, we reported an excellent hypoglycemic effect of a Ganoderma lucidium polysaccharide F31 with efficacies between 45 and 54 %, approaching to that of liraglutide (52 %). Significantly, F31 reduced the body weight gains and food intakes. F31 decreased 4 key compounds, consisting of adenosine, adenosine, galactitol and glycerophosphocholine and elevated 8 key compounds, including arginine, proline, arachidonic acid, cre-atine, aspartic acid, leucine, phenylalanine and ornithine, which protected kidney function. Also, apoptosis was promoted by F31 in epididymal fat through increasing Caspase-3, Caspase-6 and Bax and decreasing Bcl-2. On 3 T3-L1 preadipocyte cells, F31 induced early apoptosis through reducing mitochondrial membrane potential. Finally, a molecular docking was performed to reveal a plausible cross-talk between kidney and epididymal fat through glycerophosphorylcholine-Bax axis. Overall, F31 alleviated hyperglycemia through kidney protection and adipocyte apoptosis in db/db mice. This work may provide novel insights into the hypoglycemic activity of polysaccharides.