Effects of konjac glucomannan with different molecular weights on gut microflora with antibiotic perturbance in in vitro fecal fermentation

Authors：Mao, Yu-Heng; Xu, Yi-Xuan; Li, Yan-Heng; Cao, Jing; Song, Feng-Ling; Zhao, Dan; Zhao, Yimin; Wang, Zhao-Mei; Yang, Yan^*

Source：Carbohydrate Polymers, 2021, 273: 118546.

DOI：10.1016/j.carbpol.2021.118546

• Polysaccharides
• Degradation
• Protection
• Prebiotic fiber
• Fecal microflora

This study investigated the effect of konjac glucomannan (KGM) of different molecular weight on fecal microflora against antibiotic disturbance. KGM (similar to 1.8 x 10(7) Da) was partially hydrolysed with trifluoroacetic acid (TFA) for 10 and 60 min to KGM1 (similar to 2.1 x 10(4) Da) and KGM2 (7413 Da), respectively. The acid treatment caused significant reduction of intrinsic viscosity, average molecular weight (MW) and particle size of KGM, but brought limited change to the molecular structure. Low-MW KGM2 showed the most significant effect on fecal microflora in the presence of two common antibiotics (ampicillin and clindamycin), by increasing the relative abundance of Bifidobacteriaceae while decreasing the proportion of Enterobacteriaceae. Additionally, both the native and acidtreated KGM counteracted the adverse influence of antibiotics on the production of short chain fatty acids. The results have demonstrated the effect of KGM on gut microbiota with antibiotic disturbance.

• Institution
  广东药学院; y; 中山大学