Intensity Interrogation-Based High-Sensitivity Surface Plasmon Resonance Imaging Biosensor for Apoptosis Detection in Cancer

作者：Yuan, Xin; Niu, Zhenxiao; Liu, Lang; Zeng, Youjun^*; Ma, Lin; Nie, Zhaogang; Tian, Zhen; Kai, Dongyun; Zhang, Fangteng; Liu, Guanyu; Li, Siwei; Yuan, Zhengqiang^*

来源：Biosensors-Basel, 2023, 13(10): 946.

DOI：10.3390/bios13100946

• SPRi
• caspase-3
• apoptosis
• cancer
• therapeutic efficacy assessment

Intensity interrogation-based surface plasmon resonance imaging (ISPRi) sensing has a simple schematic design and is the most widely used surface plasmon resonance technology at present. In this study, we report the successful development of a novel high-sensitivity ISPRi biosensor and its application for apoptosis detection in cancer cells. By optimizing the excitation wavelength and excitation angle, we achieved a refractive index resolution (RIR) of 5.20 x 10-6 RIU. Importantly, the biosensor has been tested and validated for high-throughput and label-free detection of activated caspase-3 with its specific inhibitor Z-DEVD-FMK in apoptotic cells. Therefore, this study describes a novel molecular imaging system to monitor apoptosis in cancers for disease diagnosis and/or evaluation of therapeutic efficacy of anti-cancer drugs.

• 单位
  广东工业大学