Two-color DNA nanoprobe of intracellular dynamics

作者：Milstein Joshua N^*; Chu Mike; Raghunathan Krishnan; Meiners Jens Christian

来源：Nano Letters, 2012, 12(5).

DOI：10.1021/nl300683p

• Cellular environment
• Conformational change
• Dynamic observation
• FCS
• In-vivo
• Intracellular dynamics
• Living cell
• Microscopy technique
• Nonthermal
• Persistence length
• Single molecule
• Temporal correlation functions
• Two-color

We have developed a correlation microscopy technique to follow the dynamics of quantum dot labeled DNA within living cells. The temporal correlation functions of the labels reflect the fluctuations of the DNA nanoprobe as a result of its interactions with the cellular environment. They provide a sensitive measure for the length of the probe on the scale of a persistence length (∼50 nm) and reveal strong nonthermal dynamics of the cell. These results pave the way for dynamic observations of DNA conformational changes in vivo.

• 单位
  university of Toronto; 5; 1; 6