Sheng-Mai Yin exerts anti-inflammatory effects on RAW 264.7 cells and zebrafish

摘要

Ethnopharmacological relevance: Sheng-Mai Yin (SMY), a famous traditional Chinese medicine formula, has been commonly used in China for centuries to treat various diseases, such as inflammation-related diseases. However, the anti-inflammatory activity of SMY and its potential mechanisms still have not yet been clearly understood. @@@ Aim of the study: In this study, we aimed to determine the anti-inflammatory effect of SMY and explore its underlying mechanisms both on RAW 264.7 cells and zebrafish. @@@ Materials and methods: The levels of pro-inflammatory cytokines IL-6 and TNF-alpha secreted by RAW 264.7 cells were measured by ELISA. The protein expressions of I kappa B alpha, p-I kappa B alpha (Ser32), STAT3 and p-STAT3 (Tyr705) were determined by Western blotting. And the nuclear translocation of NF-kappa B p65 in LPS-induced RAW 264.7 macrophage cells was detected by confocal microscopy. Moreover, the in vivo anti-inflammatory effect of SMY and its potential mechanisms were further investigated by survival analysis, hematoxylin-eosin staining (H&E), observation of neutrophil migration and quantitative real-time PCR (qRT-PCR) analysis in zebrafish inflammatory models. @@@ Results: SMY reduced the release of IL-6 and TNF-alpha, inhibited the phosphorylation of I kappa B alpha and STAT3 as well as the nuclear translocation of NF-kappa B p65 in LPS-induced RAW 264.7 cells. Furthermore, the increased survival, decreased infiltration of inflammatory cells and the attenuated migration of neutrophils together suggested the in vivo anti-inflammatory effects of SMY. More importantly, SMY reduced the gene expressions of pro-inflammatory cytokines and suppressed LPS-induced up-regulation of NF-kappa B, I kappa B alpha and STAT3 in zebrafish inflammatory models. @@@ Conclusion: SMY exerts significant anti-inflammatory effects with a potential mechanism of inhibiting the NF-kappa B and STAT3 signal pathways. Our findings suggest a scientific rationale of SMY to treat inflammatory diseases in clinic.

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