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摘要
N-(2-F-18-fluoropropionyl)-L-glutamate (F-18-FPGLU), a new N-substituted F-18-labeling L-glutamate, is a potential amino acid tracer for oncology PET imaging with good tumor-to-background contrast in several tumor-bearing mice. Herein, we evaluated the potential value of F-18-FPGLU for PET imaging of glioma in orthotopic glioma-bearing SD rats. A series of competitive inhibition experiments with various types of inhibitors were conducted with C6 cells to investigate the transport mechanism of F-18-FPGLU in glioma. Establishment of orthotopic rat C6 glioma-bearing SD rats models was confirmed by MRI. Then PET imaging of F-18-FPGLU was performed on the orthotopic C6 glioma-bearing SD rats and compared with that of F-18-FDG. After the rats sacrificed, the whole brain was collected and immunofluorescence staining of glial fibrillary acidic protein (GFAP) and matrix metalloproteinase 2 (MMP2) were processed. Na+-dependent system X-AG- and Na+-independent system X-C- are the mainly transporters of F-18-FPGLU in C6 cells. N-methyl-D-aspartate (NMDA) receptor, which is associated with the invasiveness and proliferation of glioma cells, is also involved in the uptake of F-18-FPGLU. High uptake and retention of F-18-FPGLU was observerd in orthotopic glioma with good visualization and the tumor/background ratio reached 2.35 at 60 min post-injection, which was significantly higher than that of F-18-FDG (1.72) in small-animal PET images. High expression of MMP-2 and GFAP was observed in the immunofluorescence staining of glioma xerography slices. F-18-FPGLU seems to be a better potential PET tracer than F-18-FDG for brain glioma imaging with good visualization and ability to assess the tumor activity.
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单位5; 华南农业大学; 中山大学; 1; 南方医科大学
