Evidence for a molecular diode-based mechanism in a multispecific ATP-binding cassette (ABC) exporter: SER-1368 as a gatekeeping residue in the yeast multidrug transporter Pdr5

Authors：Mehla J; Ernst R; Moore R; Wakschlag A; Marquis M K; Ambudkar S V; Golin J

Source：Journal of Biological Chemistry, 2014, 289(38): 26597-26606.

DOI：10.1074/jbc.M114.586032

Background: Once an ABC multidrug transporter efflux pump expels a substrate, its reentry must be prevented.Results: Ser-1368 in Pdr5 prevents drug reflux during transport against a concentration gradient.Conclusion: Pdr5 may function as a molecular diode.Significance: The behavior of an S1368A mutant suggests an important mechanism of drug exclusion.