Comparative study on methods of DNA genotyping between single piece of dandruff and EZ-tape

作者：Hui Jian; Jing Zhu; Hui Wang; Tao Feng; Peng Bai; Weibo Liang

来源：Forensic Science International: Genetics Supplement Series , 2017, 6: e244-e245.

DOI：10.1016/j.fsigss.2017.09.097

• Dandruff
• Forensic science
• Single pieces of dandruff
• DNA genotyping

Dandruff is generally presented in criminal cases, and it contains some nucleated cells which make it possible to extract DNA for forensic uses. EZ-tape, an extraction tool for exfoliative cells, developed by Institute of Forensic Science, Ministry of Public Security, is widely used in China. Dandruff was collected by EZ-tape together with other indefinite particles, and DNA was extracted directly from EZ-tapes. In this study, we aimed to improve the utilization of samples by isolating single pieces of dandruff from 3 M scotch tapes for subsequent DNA profiling. We compared the DNA profile results of single pieces of dandruff and EZ-tape. In addition, we tried to explore whether the oscillation can affect the alleles recover. Results indicated that 56% of single pieces of dandruff with oscillation obtained full STR profiles compared with 11% with non-oscillation. Single pieces of dandruff could provide enough DNA for STR genotyping and rare sample (12.5%) could be identified mixture. Besides, both contributors are detected. However, 41.7% of samples collected through EZ-tape missed one contributor and 33.3% were mixture. Therefore, dealing with single piece of dandruff may be a potential alternative for individual identification in some special forensic casework.

• 单位
  四川大学