Immune effects of OK-432 tumor vaccine on the CD4+CD25+Treg cells of tumor mice in DAB/2 tumor-bearing mice in DBA/2 tumor-bearing mice

作者：武峰; 李琰; 田国兵

来源：中国药物与临床, 2015, (3).

DOI：10.11655/zgywylc2015.03.011

• Cancer vaccines
• Mice,inbred DBA
• T-lymphocytes,regulatory
• Flow cytometry

Objective To investigate the immune effects of OK-432 tumor cell vaccine on the CD4+CD25+Treg cells in DBA/2 mice. Methods The 7, 12-DMBA/croton oil two-stage method of carcinogenesis was used to build DBA/2 mice tumor-bearing model of skin cancer. And the tumor-bearing mice were randomly divided into three groups, which were the OK-432 vaccine group, OK-432 group and control group. The mice in each group were inject-ed with 100μl OK-432 tumor cell vaccine (5×105/100μl concentration), OK-432 (0.7 KE/100μl) and phosphate buffer saline (PBS) respectively on the left abdominal cavity once a week for continuous three weeks. The immune re-sponses of skin cancer tumor-bearing mice in different experimental groups were compared and analyzed. Results At days 1, 3, 7, 14 after the last drug intervention, the CD4+CD25+Treg cells were significantly reduced in the OK-432 vaccine group than that in the control group, with statistically significant differences ( P <0.05). Conclusion Our ex-periment confirmed that the OK-432 tumor vaccine could reduce the content of CD4+CD25+Treg cells and body im-mune suppression function.

• 单位
  山西医科大学