The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection

作者：Cheng, Chunzhen; Zhong, Yun^*; Wang, Bin; Zhang, Yongyan; Wu, Huan; Jiang, Nonghui; Wu, Bo; Lv, Yuanda; Jiang, Bo

来源：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23(13): 6971.

DOI：10.3390/ijms23136971

• citrus
• RIN4
• Huanglongbing
• disease response
• genetic transformation

The citrus industry has been threatened by Huanglongbing (HLB) for over a century. Here, an HLB-induced Arabidopsis RPM1-interacting protein 4 (RIN4) homologous gene was cloned from Citrus clementina, and its characteristics and function were analyzed to determine its role during citrus-Candidatus Liberibacter asiaticus (CLas) interactions. Quantitative real-time PCR showed that RIN4 was expressed in roots, stems, leaves and flowers, with the greatest expression level in leaves. Its expression was suppressed by gibberellic acid, indole-3-acetic acid, salicylic acid and jasmonic acid treatments, but was induced by abscisic acid and salt treatments, as well as wounding. The transient expression of a RIN4-GFP showed that RIN4 was localized in the cell membrane. RIN4-overexpressing transgenic C. maxima cv. 'Shatianyou' plants were obtained, and some transgenic plants showed greater sensitivity to CLas infection and earlier HLB symptoms appearance than non-transgenic controls. Results obtained in this study indicated that the upregulated expression of RIN4 in HLB diseased citrus may aid CLas infection.

• 单位
  y; 广东省农业科学院