Sensitive detection of MCF-7 human breast cancer cells by using a novel DNA-labeled sandwich electrochemical biosensor

Authors：Yang Yuhan; Fu Yongyao; Su Huilan; Mao Li; Chen Mei^*

Source：Biosensors and Bioelectronics, 2018, 122: 175-182.

DOI：10.1016/j.bios.2018.09.062

• Sandwich electrochemical biosensor
• DNA-labeled probe
• Au nanocage
• MWCNT-NH2
• MCF-7 cells

The simple, rapid, sensitive, and specific detection of cancer cells plays a pivotal role in the diagnosis and prognosis of cancer. We developed a novel DNA-labeled sandwich electrochemical biosensor based on a glassy carbon electrode modified with 3D graphene and a hybrid of Au nanocages (Au NCs)/amino-functionalized multiwalled carbon nanotubes (MWCNT-NH2) for label-free and selective detection of MCF-7 breast cancer cells via differential pulse voltammetry. The layer-by-layer assembly and cell-detection performance of the Au NCs/MWCNTs-NH2-based biosensor were investigated using scanning electron microscopy and electrochemical methods including cyclic voltammetry and electrochemical impedance spectroscopy. Owing to the advantages of DNA-labeled antibodies and a nanomaterial-based signal amplification strategy, the fabricated cytosensor exhibited high specificity and sensitivity when detecting MCF-7 cells in the range of 1.0 x 10(2) to 1.0 x 10(6) cells mL(-1) with a low detection limit of 80 cells mL(-1) (3 sigma/slope). Furthermore, the biosensor exhibited high selectivity when detecting MCF-7 cells and showed considerable potential for practical applications. The proposed DNA-labeled sandwich electrochemical biosensor provides a stable, sensitive approach to detecting cancer cells and is promising in terms of potential applications to cancer diagnosis.

• Institution
  成都医学院