Cytocompatibility and osteogenic activity of Ta-Ti-O nanotubes anodically grown on Ti6Al4V alloy

作者：Zhang, Meng; Xue, Yanpeng; Huang, Xiaobo; Ma, Dandan; Gao, Jie; Yu, Shengwang^*; Zhu, Liu^*; Wu, Yucheng^*

来源：Applied Surface Science, 2023, 614: 156165.

DOI：10.1016/j.apsusc.2022.156165

• Tantalum pentoxide
• Ta-Ti-O nanotubes
• Osteogenesis
• Surface modification
• Biomedical implants

Bioactive composition and bionic topography are highly desired in the surface design of biomedical implant materials. Herein, Ta-Ti-O nanotubes (NTs) were anodically grown on a tantalum (Ta) alloyed Ti6Al4V (Ta/TC4) surface. Ta/TC4 shows a scalelike surface and (alpha'+beta) microstructure with a thickness of 11.7 mu m, while Ta-Ti-O NTs exhibit a lamellar topography with -1.0 mu m in length and 48.6 nm in diameter. Ta-Ti-O NTs group has the lowest water contact angle of -16.5 degrees compared with TC4, Ta/TC4, and Ti-O NTs grown under the same electrochemical conditions. In vitro assays show that Ta-Ti-O NTs are non-cytotoxicity to MC3T3-E1 osteoblasts and slightly hamper cellular proliferation, but the adhesion and spreading abilities are significantly enhanced than others. The alkaline phosphatase (ALP), collagen type-1 (COL 1), and extracellular matrix (ECM) miner-alization levels of osteoblasts on Ta-Ti-O NTs were also improved and further confirmed by osteogenesis-related gene expressions like ALP, COL 1, OPG, and TGF-beta 1. The superior osteogenic activities of Ta-Ti-O NTs can be mainly ascribed to the combined effect of Ta2O5 doping and the lamellar distribution of NTs. In summary, Ta-Ti-O NTs film with excellent cytocompatibility and osteogenic activities will find its clinical application in the future.

• 单位
  北京科技大学