Influence of mild oxidation induced through DBD-plasma treatment on the structure and gelling properties of glycinin

作者：Liu, Zhi-Wei^*; Zhou, Ying-Xue; Tan, Yi-Cheng; Cheng, Jun-Hu; Bekhit, Alaa El -Din^*; Khaneghah, Amin Mousavi^*; Aadil, Rana Muhammad^*

来源：International Journal of Biological Macromolecules, 2022, 220: 1454-1463.

DOI：10.1016/j.ijbiomac.2022.09.127

• DBD plasma treatment
• Glycinin
• Gelling
• Oxidation
• Structure
• Functionality

The effects of dielectric-barrier discharge (DBD) plasma treatment (20 s to 120 s treatment time with 40 kV, 12 kHz) induced mild oxidation on the gelling properties, and related structural changes of glycinin were investi-gated. The gelling ability of glycinin was improved by the mild oxidation induced by the plasma treatment. Treated glycinin gels exhibited a continuous and uniform network microstructure. Samples treated for 120 s had a 2.07-, 3.99-and 2.03-fold increase in hardness, chewiness, and resilience compared to the 20 s treated samples. Structural analyses showed that primary and secondary structures of glycinin were unaffected. The tertiary structure was shifted, accompanied by a decrease in free sulfhydryl (-SH) content. At the same time, carbonyl content and average particle diameter were increased by DBD treatment. The DBD treatment facilitated the generation/exchange of intermolecular disulfide bonds and enhanced gelling properties of glycinin. It is concluded that controlled plasma-induced protein oxidation can improve protein functionality.

• 单位
  湖南农业大学