Hydrolysates of hemp (Cannabis sativa L.) seed meal: Characterization and their inhibitory effect on α-glucosidase activity and glucose transport in Caco-2 cells

作者：Cai, Lei; Wu, Shengwen; Jia, Chenggang; Cui, Chun^*

来源：INDUSTRIAL CROPS AND PRODUCTS, 2023, 205: 117559.

DOI：10.1016/j.indcrop.2023.117559

• Hemp seed meal
• Enzymatic hydrolysate
• alpha-glucosidase activity
• Caco-2 cell
• Hyperglycemia

Due to the lack of high value-added products and the rough utilization of hemp protein, which has the effect of regulating blood glucose, as well as the adverse effects of glucose-lowering drugs currently on the market, it is urgent to develop new safe and effective alpha-glucosidase inhibitors as substitutes for existing hypoglycemic drugs. Therefore, this study explored the physical and chemical properties, antioxidant activity, and inhibitory mechanism of alpha-glucosidase activity of non-dehulled hemp seed meal hydrolysate prepared by controlled enzymatic hydrolysis technology, as well as its inhibitory of alpha-glucosidase and glucose transmembrane transport in Caco-2 cells. Kinetic experiments indicated that the inhibition of alpha-glucosidase by hemp seed meal hydrolysate was reversible mixed inhibition. Cell experiments revealed that the hydrolysate inhibited both intracellular disac-charidase and the transport of glucose at different concentrations. In conclusion, the hemp seed meal hydrolysate can inhibit the activity of alpha-glucosidase and has potential value and application prospects in hypoglycemic effect.