MIR-19A ENHANCED IN HEPATOCELLULAR CARCINOMA AND REGULATED THE BIOLOGICAL FUNCTION OF HEPATOCELLULAR CARCINOMA CELLS BY TARGETING PTEN

作者：Ji, Manru; Xu, Jing; An, Hongjun; Fu, Zhongze; Nie, Wenbao; Shi, Yanhui; Wei, Dongmei^*

来源：Acta Medica Mediterranea, 2021, 37(3): 1749-1753.

DOI：10.19193/0393-6384_2021_3_278

• miR-19a
• PTEN
• liver carcinoma
• proliferation
• invasion
• targeted regulation

<bold>Objective</bold>: To investigate the content of miR-19a in hepatocellular carcinoma (HCC) and its role on the biological function by targeting PTEN. @@@ <bold>Methods:</bold> miR-19a in 85 patients with liver carcinoma was tested by qPCR, and the content of miR-19a in HCC cells (HepG2, SMMC7721, BEL-7402, Hep3B) and human healthy liver cells (HL-7702) were tested to select suitable experimental cells. The role of miR-19a on the proliferation of HCC cells was determined using CCK-8 and clone formation test. Transwell test and WB test were applied to test the invasion of HCC cells and the content of related invasion proteins. The correlation of miR-19a with PTEN in HCC was detected using luciferase and Western blot. <bold> </bold>Results: miR-19a was enhanced in HCC. CCK-8 and clone formation test revealed that the proliferation of SMMC7721 transfected with miR-19a inhibitor was obviously weakened. Transwell suggested that decrease of miR-19a in HCC cells could weaken the invasion of HCC cells, and E-cadherin protein enhanced evidently, while Vimentin protein reduced. Flow cytometry suggested that the apoptosis of miR-19a inhibitor enhanced evidently. Double fluorescein report suggested that PTEN was the target of miR-19a. <bold> </bold>Conclusion: miR-19a may affect the proliferation, invasion, and apoptosis of HCC cells by elevating PTEN.

• 单位
  1; 南方医科大学; 6