Galvanic replacement reaction-regulated photoelectric response and enzyme-mimicking property of ionic liquid functionalized Cu@Cu2O aerogels for dual-mode immunoassay

作者：Li, Jinli; Wang, Hengjia; Liu, Mingwang; Qin, Ying; Tan, Rong; Hu, Liuyong^*; Gu, Wenling^*; Zhu, Chengzhou^*

来源：Chemical Engineering Journal, 2023, 455: 140743.

DOI：10.1016/j.cej.2022.140743

• Dual -mode biosensing
• Photoelectrochemical biosensing
• Nanozymes
• Colorimetric biosensing
• Galvanic replacement reaction

Prostate-specific antigen (PSA) is a characteristic serum biomarker for screening prostate cancer, which benefits the prostate cancer diagnosis at an early stage. At present, the reported strategies for PSA detection are mainly simplex signal readout, inevitably limiting the inherent accuracy and reliability of the involved bioassay. In this work, ionic liquid functionalized Cu@Cu2O aerogels (IL-Cu@Cu2O) with the photoelectric response and enzymemimicking activity are used to construct a dual-mode biosensing platform toward PSA with Ag nanoparticles as the signaling labels and amplifiers. After the sandwich immunocomplexing, Ag+ ions could be subsequently released and directly interact with IL-Cu@Cu2O aerogels via galvanic replacement reaction, resulting in the enhanced photoelectrochemical (PEC) signal and decreased enzyme-mimicking activity for dual-mode signal readout of the PSA concentration. As a result, the proposed strategy presents wide detection ranges of 0.1 pg/mL to 5 ng/mL (PEC) and 5 pg/mL to 1 ng/mL (colorimetric) and ultralow limits of detection of 0.04 pg/mL (PEC) and 0.63 pg/mL (colorimetric) for PSA, which provides great prospects for highly accurate disease diagnosis in practical application.

• 单位
  武汉工程大学